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[9000963.] رقم البحث : 9000963 - |
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In vitro Propagation of Four Grape Cultivars / |
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قطاع الدراسات الزراعية / الفاكهة |
| تخصص البحث : الفاكهة |
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Assiut Journal of Agricultural Sciences / / Vol. 46, no. 4 - December 2015 |
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يوسف خيرى انور ( 01226313930 ) - مؤلف رئيسي |
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in vitro propagation, grape cvs., shoot tips, nodal segments: |
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Successful propagation of in vitro four grape cvs. (Concord, Thompson Seedless, Beauty Seedless and King Ruby) through shoot tips and nodal cuttings was established. The best explants sterilization was achieved with 10% cholorox for 10 minutes. The combination of 1.0 mg/L BA and 0.01 mg/L NAA produced the maximum sprouting rate (100, 91.7, 100, 83.3%) and the least number of days for buds sprouting (6, 9, 5 and 11) of Concord, Thompson Seedless, Beauty Seedless and king Ruby cultivars, respectively. Additionally, the same medium induced the greatest average of shoots number/explants (5.50, 4.95, 5.27 and 4.69) and the highest shoot length (5.3, 5.2, 5.2 and 4.9 cm) for the four tested grape cvs. The third subculture induced the maximum response of sprouting fre-quency and the optimum average of shoot numbers/explants for the four exam-ined cultivars. For rooting of in vitro shoots, Half strength MS medium supplied with 1.0 mg/L IBA resulted in the best root formation (90, 100, 80 and 70%) for Concord, Thompson Seedless, Beauty Seedless and King ruby cultivars, respec-tively. |
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