الفهرس 
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Abstract
Meat products serve as very good medium for the growth of many microorganisms including Staph. aureus causing public health hazards and economic losses.
A total of 120 random samples of minced meat, luncheon and pastirma (40 of each) were collected from the super markets located in Sohag city, Egypt for the isolation and identification of Staph. aureus.
The samples were submitted for conventional and molecular examination by traditional and molecular methods. Cultivation on culture media, and biochemical reactions represented the conventional methods while amplification of 23S rRNA gene represented the molecular identification method.
The obtained results showed that Staph. aureus was detected in thirteen samples from totally collected one hundred and twenty meat products samples (10.8%), Staph. aureus incidence in the examined mined meat, luncheon and pastirma samples were 20% (8/40), 10% (4/40) and 2.5% (1/40), respectively.
Regarding PCR confirmation, Staph. aureus isolates were examined for detection of 23S rRNA gene. All examined strains were positive for the 23S rRNA gene.
The influence of cinnamon essential oil and nisin and their nano-materials on the viability of Staph. aureus was detected. In addition, their nano-materials were prepared and characterized. The average droplet size and PDI of nanomaterials were measured by Zetasizer and the PDI were 0.234 and 0.197 for CNE and NNP, respectively. PDI results indicated the stability of the prepared nano-materials. As well as the average droplet size were 352.8 nm and 99.32nm for CNE and NNP, respectively.
Furthermore, the morphology of the nano-materials was visualized by TEM, the image revealed that most of the nano droplets were almost spherical in shape, with ranged size of 16.9-19 and 11-14.7 nm for CNE and NNP, respectively.
The MIC of the prepared cinnamon oil and nisin and their nano-materials were detected in-vitro against the isolated Staph. aureus strains by using agar well diffusion method, in which, the results revealed that the MIC were 0.39% for CEO, nisin and NNP, and 0.78% for CNE.
The different concentrations of cinnamon oil and nisin and their nano-materials have a great effect against Staph. aureus growth and the most effective one at the MIC (0.39%) was nisin with 21.67 mm.