الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
”Burn is as demolition found in the epidermal tissue, deeper tissues or dermal tissue and this injury caused to the skin or other tissues caused by heat, cold electricity, chemical, friction or UV rays such as (sunburn). The infected burn is the biggest problem that faces any country. Infections caused by antibiotic-resistant bacteria have been declared as a global threat to public health or through infection from hospitals. The overuse of antibiotics over a long period has allowed infectious organisms to adapt to antibiotics and, in turn, reduce their effectiveness, and reduce immunity of special cases such as immune-compromised patients also high cost of it. In this study use alternative therapy to treat the burn (wound injury) infection as safe as food it’s probiotics. These are effective compounds in the treatment and protection of some alimentary track infections as they alter the intestinal flora in favor of beneficiary Microorganisms. Lactic acid bacteria (LAB), is of interest because they are active in a nano molar range, do not have toxic effects, and are readily available in fermented food products. The aim of this study was to evaluate the antibacterial, anti-inflammatory effects and wound healing process has been tested for Lactobacillus filtrate and lyophilized cell solution. The in vitro anti-inflammatory action was tested using lipoxygenase enzymatic inhibition assay where lyophilized cells solution sample, showed good inhibition of lipoxygenase action with IC50 value 13.3±2.1 µg/ml, while IC50 =12.7±1.9µg/ml for filtrate sample versus standard drug IC50 value of 1.3±0.7 µg/ml. Agar well diffusion assay was also performed to test the antibacterial action against standard bacterial strains (Pseudomonas aeruginosa , Staphylococcus epidermis, Candida albicans, E- coli and staphylococcus aureus) . Moreover, crude metabolites of Lactobacillus sample had a significant antimicrobial activity among different bacterial strains. The highest inhibition zone value (20.2±1.8 mm) of metabolites of Lactobacillus sample was observed for S. aureus strain. In vivo study using Lactobacillus filtrate and lyophilized cells solution were applied in infected wound with S. aureus to compare their healing activities versus chloramphenicol. It could be noticed that both filtrate and lyophilized cells solution has a successive impact after application to infected wound compared to treatment using chloramphenicol which enhance clinical status of animals and positive impact body weights as well as examined healing activity using diameter of wound. In the present work after application of filtrate and lyophilized cells solution of Lactobacillus sample in infected wound, bacterial load in skin were disappeared completely in skin, liver and spleen of animals treated with filtrate, while a minimal bacterial load could be seen in skin and disseminated to internal organs upon using lyophilized cells solution in a similar level relative to using chloramphenicol as microbicidal agent.. In the histopathological sections, uninfected rats had a healing capacity about 90%. However Lactobacillus filtrate group and the lyophilized cells group were able to accomplish about 90% and 80% healing, respectively, in wound-infected animals. This suggested that Lactobacillus filtrate might function as a dual mechanism to aid in skin regeneration and infection eradication. Also, Lactobacillus filtrate, or lyophilized cells, or chloramphenicol in various treatments lead to optimize levels of protective mediators including, TNF-α, and IL-17 as well as up regulation of level of anti-inflammatory cytokine (IL-4) which reported to have roles in both autocrine and paracrine mechanisms, encourage the recruitment, growth, and development during healing process. At the end, this study suggest the possibility of using Lactobacillus sample or its metabolites in wound healing in wound infection model with effective antibacterial, anti-inflammatory actions and minimal side effects on kidney and liver functions to be produced in large scale after further pharmacological studies.