الفهرس 
REVIEW OF LITERATUREOnly 14 pages are availabe for public view
 |  | from | 253 |  |  |
| | | from | 253 | | |
Abstract
The present study was established to investigate the role of bone marrow mesenchymal stem cells (MSCs) and (EA for improvement the functions of kidney and liver in VEN-induced renal and hepatotoxicity.
Seventy male albino rats were divided into seven groups:
GI (control): Received distilled water daily.
GII (Antidepressant drug toxicity model): Received Venlafaxine (VEN) orally (18 mg/kg/day) for 8 weeks (Bielski et al., 2004 and Reagan-Shaw et al., 2008).
GIII: Received VEN (18 mg/kg/day) oral gavage for 8 weeks then left for 4 weeks.
GIV : Received EA orally (100 mg/kg b.w./day) for 8 weeks (El-Shitany et al., 2014).
GV: Received EA (protective) orally at dose (100 mg/kg/day) for 4 weeks then receives both EA + VEN (18 mg/kg/day) oral gavage for 8 weeks.
GVI: Received VEN (18 mg/kg/day) oral gavage for 8 weeks then EA orally at dose (100 mg/kg b.w./day) for 4 weeks (Bielski et al., 2004 and Reagan-Shaw et al., 2008).
GVII: Received VEN (18 mg/kg/day) oral gavage followed 8 weeks later by i.v. injection of single dose labeled BM- derived mesenchymal stem cells (3 x 106 cells per rat) (Dong et al., 2008). After two months, rats were sacrificed.
The present results recorded a significant decrease in body weight and highly significant increase in organ weights in GII compared to GI. Treatment of VEN rats with MSCs alone or with EA significantly increased body weight and enhanced in organ weights when compared to VEN- group (GII). A highly significant elevation of kidney functions, liver functions and lipid profile markers were observed in VEN-group (GII) compared to GI. While, a marked significant reduction of kidney functions, liver functions and lipid profile markers were observed in GII when treated with MSCs more than with EA alone. Also, total protein, albumin and antioxidant parameters were recorded in the same groups where, detected by significant elevation of serum MDA level concomitant with significant reduction of serum GSH and SOD, total protein and albumin in GII compared to GI. Treatment of VEN with MSCs alone or with EA improved the levels of GSH, SOD, MDA total protein and albumin to the values close to the control level.
VEN-treated rats showed marked inflammation as indicated by high levels for the tumor necrotic factor-α and CRP as well as low level of serum cytokine IL-10. The inflammation induced by VEN was reduced under the effect of MSCs treatment or with EA.
The histological examinations showed different pathological injuries degrees in liver and kidney tissue as shown in VEN treated GII while in recovery group slightly improvement appear. In protective group markedly improvement in both of liver and kidney tissue but in the GVI which received VEN for 8 weeks then EA for 4 weeks showing slightly improvement. Finally using MSCs in GVII showing regenerated hepatic and renal tissue.
It can be concluded that MSCs showed a highly improvement in all studied parameters based on the morphological, biochemical, and histological investigations.