الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 134 |  |  |
| | | from | 134 | | |
Abstract
Interstitial cystitis” (IC) is A persistent inflammatory pain condition known as primarily affects women and causes discomfort in the bladder and pelvis. A feeling of pressure and discomfort in the bladder, along with symptoms of the lower urinary tract that last longer than six weeks, are used to diagnose it. The presence of Hunner’s ulcer or mucosal haemorrhages in the bladder (glomerulations) was used to diagnosis IC in the absence of disease or other factors. Later, a non-ulcerative variant of IC was discovered and characterised in more than 90% of all diagnosed individuals with IC. Here in this study, authors evaluated the gene expression of leucine rich pentatricopeptide repeat (LRP130), sirtuin 7 (SIRT7) and sirtuin 5 (SIRT5) by real time polymerase chain reaction (RT-PCR) in IC affected bladder tissue and normal bladder tissue and determination levels of the oxidative markers as nitric oxide (NO) and protein carbonyl (PC) and catalase (CAT) and superoxide dismutase (SOD) were evaluated as antioxidant marker by spectrophotometric assays in serum and urine of IC and Healthy control groups. Current study involved 40 female participants, (27) IC patients and (13) healthy control. All patients’ surgical and medical history were taken and they were given a physical examination. Blood, urine and tissue samples were collected. The following results were obtained: • The levels of nitric oxide (NO) in serum and urine were significantly higher in IC group compared to control groups. • Levels of protein carbonyl in urine was significantly increased in IC group compared to control groups. • The levels of catalase (CAT) and superoxide dismutase (SOD) were showed non-significant difference between IC and healthy groups. • Moreover, the fold of change of the gene expression of leucine rich pentatricopeptide repeat (LRP130) and sirtuin 7 (SIRT7) was higher in the ulcer tissue than the normal. Whereas the fold of change of the gene expression of sirtuin 5 (SIRT5) was lower in the ulcer tissue than the normal tissue. • Serum NO level was positive correlated with age. Likewise positive correlation between LRP130, NO and SIRT7. • Moreover, negative correlation was observed between serum SOD and LRP130 fold of change. Conclusions and recommendations: from the current study, we can conclude that, the levels of oxidative stress markers are elevated in both serum and urine of IC patients. This elevation may be due to the pathogenic role of free radicals in the disease both on genetic and molecular levels. In addition, SIRT5 and SIRT7 play a fundamental role in regulation of oxidative stress on the bladder cells induced IC. Moreover, signaling pathway of PI3K/AKT/mTORC1 in IC patients leads to overexpression of LRP130 and may represent a novel pathway for development and pathogenesis of IC. Further research is needed for studying free radicals as possible theory in the pathogenesis of IC and possible protective role and treatment trials of antioxidant and for investigation the association of LRP130 with oxidative stress to facilitate the diagnosis and treatment of IC.