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العنوان
DNA BARCODING AND RELATIONSHIPS
OF SOME LADYBIRD BEETLES:
المؤلف
Altayef, Hanan Hussien.
هيئة الاعداد
باحث / حنان حسين الطيف
مشرف / رمضان أحمد محمد على
مشرف / أحمد صابر محمد بريم
مشرف / محمود مجدي عبد الله عوض
تاريخ النشر
2023.
عدد الصفحات
104 P. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
Molecular Biology
تاريخ الإجازة
1/1/2023
مكان الإجازة
جامعة عين شمس - كلية البنات - قسم علم الحيوان
الفهرس
Only 14 pages are availabe for public view

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from 104

Abstract

The Northern Coast of Egypt “El Sahel” or ”The Egyptian Coast extends for about 1,050 km along the Mediterranean Sea covers entirely the northern territory of Egypt and is considered one of the longest Mediterranean coastlines in North Africa. A
one of the natural highly diverse spots, it was the sampling location of the ladybird beetles of the current study. The study included morphological characterization as well as a brief description of the surveyed species. The current study aimed to include a molecular description and identification sequences to enrich the database with the collected individuals of ladybird beetles. Such information is essential for scientific purposes to study the species diversity and understand their predatory behavior and their potential biological control ability. The application of mitochondrial cytochrome oxidase subunit I to the DNA barcoding technique was evolutionary and caused a relevant contribution to the databases of battles around the world, with a straightforward approach that is considered an easy to use, efficient and cost-effective tool for identification as well as most of the known organisms. A total of 16 samples, were sampled, and barcoded using the mt-COI gene in were successfully sequenced and identified.
Most of the species showed the correct name, while some showed a deviation to a proximate species similar in phenotype or shows a clear confusion due to multiple local nomenclator present. establish the DNA barcoding protocol for further and expanded Therefore, other two genes the small subunit rDNA 18S, and the rRNA 28S were sequenced to confirm the taxonomical position of the identified species. The current study was planned to surveys to identify ladybird beetles to enrich the international database with some of the species located in this area.