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Abstract
Background & objectives: Paracetamol (acetaminophen) overdose is one of the most common causes of acute liver injury in the world. Early detection of drug induced liver injury remains a problem as the classic markers for liver injury lack clinical sensitivity and specificity. MiRNA 122 & miRNA 192 are promising biomarkers with high sensitivity and specificity for detection of drug induced liver injury. Aim of this study: The aim of the current study is to investigate the potential utility of miRNAs 122 & 192 as markers of human paracetamol-induced liver injury and to compare miRNA with existing biomarkers of liver injury in humans. Results: The age of all participants ranges between 11-57 years with mean age in group (1) 24.83, in group (2) was 23.2 and in group (3) it was 31.07. We found that 100% of cases were suicidal. Males were 21.4% and females were 78.6% of all studied cases. The mean and standard deviation of paracetamol level (mg/l) in group (1) was 287.34±79.74 and in group (2) it was 31.71±12.67. The mean and standard deviation of liver enzymes (AST & ALT) (IU/L) in the study group (1) was in normal range (31.31±2.71 & 20.57±1.96 respectively). The mean and standard deviation of liver enzymes (AST & ALT) in the study group (2) was in range higher than normal limits (91.80±26.93 & 39.29±13.77respectively). Results of paracetamol level, miRNA 122 levels and miRNA 192 levels showed statistical significance between the study group G3 and both groups of cases (groups G1 & G2). Conclusion: Both types of miRNAs included in the study (122 & 192), are highly sensitive markers of paracetamol induced liver injury in cases both with early and late presentation. Keywords: paracetamol, miRNA, RT-PCR, Drug induced liver injury