الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Diabetic nephropathy (DN) is a progressive kidney disease, and it is estimated to develop in one third of both main types of diabetes. New biomarkers are needed to improve the identification of individuals at increased risk of developing diabetes mellitus complications particularly DN. The current study aimed to evaluate the diagnostic potential of two circulating microRNAs (miR-92a and miR-346) as a non-invasive biomarker for DN in type 2 diabetes mellitus Egyptian patients. A total of 90 plasma samples were collected (15 control, 25 normoalbuminuria patients and 50 patients with DN that divided equally into microalbuminuria group and macroalbuminuria group, n= 25). The expression levels of the two miRNAs were quantitatively detected using real-time PCR. Results revealed significant decrease in miR-346 expression level, while miR-92a expression level was significantly upregulated in the DN groups compared to control. Correlations between the studied miRNAs levels and the tested biochemical parameters was conducted, in addition to receiver operating curve analysis to detect the potential application of miR-346 and miR-92a for discriminating diabetic nephropathy patients from non-diabetic nephropathy patients. Higher sensitivity and specificity of plasma miR-92a than miR-346 was observed. In conclusion, miR-92a and miR-346 might be a potential non-invasive biomarker for DN with miR-92a showing privilege over miR-346 that should inspire additional research.