الفهرس 
Hematopoietic Stem Cell Transplantation, An overviewOnly 14 pages are availabe for public view
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Abstract
Based on our study we concluded that CD146 could have a therapeutic potential if infused in patients with PGF as it may help the process of recovery from the PGF state based on its ability to increase stem cell proliferation and improve the patients’ survival.
Recommendations
According to our study, we recommend that CD146 can have a potential therapeutic role if CD146+ selected mesenchymal cells are infused in patients with refractory poor graft function to enhance the proliferation of stem cells and help in recovery of patients. However further studies are warranted to support its suggested therapeutic role.
Summary
For some benign or malignant hematopoietic diseases, allogeneic hematopoietic stem-cell transplantation (allo-HSCT) is crucial and the only curative treatment available. The outcome of the graft is affected by a number of variables, including the bone marrow microenvironment.
The current study was conducted to assess how the bone marrow microenvironment is being affected in patients with poor graft function post allogenic stem cell transplantation targeting the perivascular mesenchymal cells using CD146 antibody.
Forty patients were enrolled in our study with both benign and malignant hematological diseases. Patients were divided into two groups: group 1 includes 20 patients with poor graft function, group 2 includes 20 patients with good graft function
Patients were subjected to full history taking, full laboratory work-up then bone marrow aspirate was performed for histological testing and measuring CD146+ stem cells by multiparameter flow cytometry.
Analysis of demographic data and clinical characteristics revealed that sex mismatching, ABO incompatibility and major mismatch were significantly associated with PGF.
Analysis of hematological data revealed that PGF group showed significantly lower counts of WBCs, platelets, hemoglobin concentration and CD34+ cells compared to GGF group in addition to the presence of hypocellular marrow seen in the bone marrow examination post-transplant.
According to the analysis of percentage of stem cells with specific markers revealed that PGF group had statistically significant lower percentage of total count of stem cells compared to GGF group. Concerning CD146 expression on stem cells, it was statistically higher in PGF group in comparison to GGF group. On the other hand, as regards the expression of CD146 by the non- stem cells, the MFI of CD146 expression was significantly higher in PGF group compared to the GGF group, but the percent of CD146+ non stem cells showed only borderline higher significant expression in the PGF group.