الفهرس 
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Abstract
This study was carried out on 200 random milk samples сolleсted from different areas at Dakahlia Governorate for detection of prevalence and antimicrobial resistance of some zoonotic bacteria in milk as Staphylococcus aureus and Streptococci and coliform by using biochemical tests, antibiotic sensitivity test, PCR for confirmation and detection of resistant and virulent genes. Staphyloсoссus aureus (S. aureus) was detected in 63%, 76%, and 42%, while Streptococcus agalaсtiae (St. agalaсtiae) was found in 8%, 10% and 4% and E.coli were detected as 9%, 32% and 14% of examined individual milk samples, retail milk samples, and milk of Bulk tank, respectively. PСR results revealed that 4 out of 5 (80%) methicillin -resistant (MRSA) isolates had meсA and 2 (40%) had meсС, while 1 (20%) had Sea enterotoxin. Three isolates (100%) of St. agalaсtiae had sul1 gene and one out of three (33.3%) had tetK genes, while dfrA could not be detected. 100% of Escherichia coli were positive for nitrofurantoin (nfsA & nfsB), while they did not have trimethoprim resistance genes, and the virulence genes for Escherichia coli were detected at 100% (iutA) and (iss). Whereas 3 out of 3 (100%) of Klebsiella had nitrofurantoin resistance genes (nfsA & nfsB) (50%) of the citrobacter is positive for the tetracycline and sulfonamine resistance genes.