الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
Brucellosis is one of the most important risky and complicated zoonotic, public health and economic problems that came in the foreground of epidemiological studies all over the world. it is called undulant fever, Mediterranean fever or Malta fever. It affects both human and animals through ingestion, direct contact with infected animal or its products or through inhalation of infectious aerosols The aim of this study is to: Investigate the occurrence of brucellosis in cattle and sheep. Investigate the occurrence of brucellosis in human. Molecular detection of Brucella species from aborted foeti, spleen, lymph nodes and milk of seropositive animals. Materials & methods: A total of 868 animals (630 cattle and 238 sheep) were used in this study. Concerning cattle, (250 from benisewef governorate and 380 from Damietta governorate). However 238 sheep (39 from Benisewef and 199 from Elmonofia) were investigated in this study. 180 milk samples were taken from 180 seropositive cows. The collected milk samples were divided into two parts. One part subjected to Milk Ring test (MRT). Another part kept frozen till assessed by Real time PCR. 1.Rose Bengal plate test: The test was carried out according to. The sera under test and antigen were brought to room temperature before testing. Using antigen micropipette, a DROP (30ul) of the serum was placed on dry antigen (30ul). Buffered acidified plate agglutination test (BAPAT: The test was performed according to method mentioned previously by The sera to be examined and the antigen were stored at room temperature before the examination, then 80 μl of every sample were put on the centre of a square-etched area of the double-strength glass plate of the Minnesota testing box using an 80 µl.-fixed pipette. 3 complement fixation test: Was carried out as mentioned before. 4.Milk ring test: MRT was performed for the milk samples, which were positive for abovementioned three serological tests. Briefly, 0.1% formalin or 0.02% bronopol (as a preservative) were added to the samples at 4°C for 2–3 days before the usage if it is mandatory. 5.Rose Bengal Plate Agglutination Test (RBPAT): A total of 125 human sera was assessed by RBPAT for anti-Brucella antibodies as mentioned in occurrence of brucellosis in cattle and sheep. 6.Tube agglutination test: Was A total of 125 human sera was as tested by TAT. The test was carried out according to Complement Fixation Test (CFT): A total of 125 human sera were confirmation of brucellosis by CFT as carried out as in animals. Recommendations: Routine and regular regime of serological tests must be applied for cattle farm and sheep herd, aborted one must be intensively research for the cause. Aborted foeti, placenta and contaminated material must be hygienically destroyed either by burning or deep burial. The premises must be thoroughly disinfection by suitable disinfectants. Use of artificial insemination to minimize infection.