الفهرس 
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Abstract
The objective of this study is to investigate in vitro the feasibility of using AC treatment in infected root canals with complex microbes biofilm, which is a major challenge in endodontic treatment.
A total of Ninety extracted human permanent maxillary central incisors with matured apices were selected, decoronated at CEJ and classified according to the main intracanal medicaments into 3 main groups (n=30). Each GP was further subdivided into 3 subgroups (n=10), according to the period of application, first subgroup (n=30) was evaluated after 24hours, second subgroup (n=30) was evaluated after one week, and third subgroup (n=30) was evaluated after two weeks.
A complex microbe biofilm suspension was developed and used to infect the samples. The infected samples were then incubated. Colony forming units were recorded before and after the application of the intracanal medication. The antibacterial effectiveness of different intracanal medicaments (AC, CaOH, AC+CaOH was evaluated by counting colony forming units and determining the percentage reduction in colony counts (%RCC) at the end of each time period. The results were compared and statistically analyzed.
Results showed that after 24hr, 1 week and 2 weeks of incubation, there was a significant reduction in the mean of the colony forming units after using AC or AC+ CaOH when compared with CaOH control group.
Conclusions:
Under the circumstances of this study, it can be concluded that:
A. Activated charcoal can be incorporated into intracanal medicament paste for elimination of bacterial biofilm.
B. Addition of CaOH to activated charcoal can potentiate the antibacterial effect.
C. Activated charcoal has the potential to play a big role in endodontic treatment in the future.
Recommendations:
Further studies are needed to determine the other effects of the combination of CaOH and AC on the root canal dentine.