الفهرس 
Pathophysiology of cancerOnly 14 pages are availabe for public view
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Abstract
Large scale screening of selected Egyptian plant extract’s
against human colon, liver, breast and lung carcinoma cell
lines for detection of antitumor activity in vitro.
Key words: Egyptian plants, Cell lines, Antitumor, In vitro
Forteen plants belonging to 6 families were collected from different
areas in Egypt. These plants yielded 50 methanol extracts and were tested
for their anticancer, free radical scavenging and anticandidal activities.
The anticancer activity was tested against four human cancer cell
lines, namely, A549 (lung carcinoma), HCT-116 (colorectal carcinoma),
HepG2 (hepatocellular carcinoma) and MCF-7 (breast adenocarcinoma).
Cell viability was determined using the MTT (3-(4, 5-dimethylthiazol-2-
yl)-2, 5-diphenyltetrazolium bromide) assay. Free radical scavenging
activity was tested using 2, 2 diphenyl-1-picrylhydrazyl (DPPH) assay.
Anticandidal activity was measured using disk diffusion method.
Results showed 11 methanol plant extracts possessed both high
cytotoxicity and free radical scavenging activities, these included: branch
extract of Anacardium occidentale,bark extract of Pleiogynum solandri,
branch extract of Pistachia terebinthus, leaves extract of Harpophyllum
caffrum, leaves extract of Albizzia stipulate, branch extract of Derris
robusta, bark extract of Bauhinia variegate, bark extract of Adenanthera
pavonina, branch of Albizzia stipulate, leaves of Derris elliptica and bark
of Hyphaene thebaica. On the other hand, 14 methanol plant extracts
possessed high free radical scavenging activity and showed weak
cytotoxicity against the tested cancer cell lines. All these promising
extracts showed no activity against candida albicans except the branch
extract of Albizzia stipulate which possessed moderate anticandidal
activities. Leaves and fruit extract of Aegle marmelos showed
anticandidal activity but did not possess neither cytotoxic nor free radical
scavenging activities. These promising extracts were further tested for
cytotoxicity on human normal cell line (BJ-1) to calculate their selectivity
index (SI) values. In vitro cytotoxic effect on 3D cancer spheroids were
carried out for the most active plant extracts to select the most active to
be subjected to initial fractionation. Six extracts showed moderate to high
cytotoxic effect on 3D cancer cells: the leaves extract of Albizzia
stipulate, bark extract of Hyphaene thebaica, leaves extract of
Harpophyllum caffrum, branch extract of Pistachia terebinthus and
Albizzia stipulate and leaves extract of Derris elliptica. These extracts are
considered the most promising for anticancer activity in the context of
this study and deserve further chemical and biological investigations.