الفهرس 
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Abstract
The currentwork studied the relation between blood serum cholesterol of volunteers and colonies number of normal intestinal micro flora isolated from feces and cultured on selective media as MRS for lactic acid bacteria and SDA for yeast.Stool and blood serum were collected from 80 volunteers their age ranged from one month to 70 years those divided into four different groups according to their age, from one month to 2 years ,from 2 to 20 years ,from 20 to 40 years and finally from 40 to 70 years. The First group were children feeding on dairy products which contain high lipids but blood serum cholesterol level showed normal range because of high colonies number of yeast cells and lactic acid bacterial cells . The other groups showed that the increase in microbial colonies count for lactic acid bacteria was inversally proportional and statically significant with blood serum cholesterol level.The isolated andtested yeast and bacterial isolates were tested for their ph. and bile salt tolerance . The amount of cholesterol removed by viable and dead yeast cells Saccharomyces cerevisae confirmed that there was significant difference between cholesterol removal by viable and dead cells, cholesterol removal, by viable cells ranging from 1.4% after incubation 12 hours , 3.3% after incubation 24 hours, 5.3 % after incubation 36 hours,, and 7.87 % after incubation 48 hours , cholesterol removal, by dead cells ranging from 0. 59% after incubation 12 hours , 1.5 % after incubation 24 hours, 2.36 % after incubation 36 hours,, and 3.18 % after incubation 48 hours. The amount of cholesterol removed by viable and dead Bacterial cells of L. acidophilus showed a significant difference between cholesterol removal by viable and dead cells, cholesterol removal, by viable cells ranged from 0. 47% after incubation 12 hours , 1.3% after incubation 24 hours, 2.59 % after incubation 36 hours,, and 4.3 % after incubation 48 hours, cholesterol removal by dead cells ranging from 0. 2% after incubation 12 hours , 0.5% after incubation 24 hours, 0.99 % after incubation 36 hours,, and 1.6 % after incubation 48 hours. In rats treated with yeast blood serum cholesterol decreased by 5% after 14 days from beginning the treatment .10% after 28 days and decreased by 24.74% after 42 days from beginning of treatment In rats treated with yeast bacterial cells, blood serum cholesterol decreased by 8.06% after 14 days from beginning the treatment .13.18% after 28day and decreased by 11.85% after 42 days from treatment . Rats treated with mixed ( Saccharomyces & lactobacillus ) blood serum cholesterol decreased by 4.19% after 14 days from beginning the treatment 10.39% after 28 days and decreased by 14.5% after 42 days from treatment. Also, our study showed that there was significant difference between amount of cholesterol assimilation when rats treated with Bacterial cells L. acidophilus alone and Saccharomyces cerevisae , significant difference between L. acidophilus as alone and L. acidophilus mixed with Saccharomyces cerevisae , and there was not significant difference between amount of cholesterol assimilation by Saccharomyces cerevisae as alone and L. acidophilus Inrats treated with yeast cells blood serum triglycerides decreased by 3.16% after 14 days from beginning the treatment 5.53% after 28 days and decreased by 12.94% after 42 days from treatment . When rats were treated with bacterial cells blood serum triglycerides decreased by 6.32% after 14day from beginning the treatment 10.38% after 28 days and decreased by 16.9% after 42 days from treatment. when rats were treated with mixed ( saccharomyces & lactobacillus ) blood serum triglycerides decreased by 3.16% after 14 days from beginning the treatment 5.82% after 28 days and decreased by 16.24% after 42days from treatment . Also, our results showed that there was not significant difference between Triglycerides decreased when rats treated withBacterial cells L. acidophilus as aloneand Saccharomyces cerevisae ,andL. acidophilus mixed with Saccharomyces cerevisae. Rats treated with diet containing high cholesterol and treated without yeast and bacterial supplementation either individually or together showed abnormal RBC,s membrane because of the deposition of lipid particles . On the other hand ,rats treated with diet containing high cholesterol and treated with yeast and bacterial supplementation either individually or together showed normal RBC,s with much less lipid particles deposition on membranes according to treatment. Also, electron microscope studies were performed for yeast and bacterial cells treated and grown in cholesterol confirming the possibility of absorption and adsorption of cholesterol particles by yeast and bacterial cells Our current study has given explanation of and the reasons for widespread use of probiotics for improving the lipid profile (total cholesterol, LDL-cholesterol, HDL-cholesterol and triglyceride) using rats as an experimental models . Certain strains of probiotics have demonstrated cholesterol lowering properties while others did not. Cholesterol assimilation by viable cells was significantly higher than dead cells counterparts. The capability of microorganisms to remove cholesterol in dead stage indicated that cholesterol might also be removed via binding to cells. Higher cholesterol removal by growing cells indicated that the degree of bound cholesterol might be dependent on the growth of cells.