الفهرس 
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Abstract
This study was carried out at International Livestock Management Training Center (ILMTC), Sakha, Kafrelsheikh Governorate, belonging to the Animal Production Research Institute, Ministry of Agriculture, Agricultural Research Center, Egypt, during the period from August 2009 to March 2011.
A total of 30 New Zealand White (NZW) and 30 Baladi Black (BB) rabbit does was used as donors of oocytes as well as 6 fertile bucks, (3 NZW and 3 BB) were used for semen collection of achieving in vitro fertilization in this study.
The present study included three experiments, oocyte recovery and in vitro maturation; semen collection, sperm capacitation and in vitro fertilization.
In conclusion, the present study indicated that:
1- There were significant differences between NZW and BB in ovarian weight and number of follicles or oocytes per ovary or doe, being significantly higher in BB than in NZW breed However, oocyte recovery rate was not affected by rabbit breed, being 88.34 and 90.16% in NZW and BB breeds.
2- Supplementation of TCM-199 with AA had a beneficial effect on in vitro maturation of rabbit oocytes; and that can be achieved at levels of 2 mM for NZW and 3 mM supplemental AA for BB oocytes.
3- Semen collected from BB bucks was of good quality than that obtained from NZW bucks under the Egyptian condition.
4- Heparin supplementation at a level of 35μg/ml had beneficial effect rather than other heparin levels or all calcium ionophor levels on sperm capacitation used for in vitro fertilization for NZW and BB breeds.
These findings may indicate the beneficial effects of antioxidant supplementation of in vitro maturation medium with different sources and levels on in vitro fertilization rate of BB and also NZW breed rather than effects of antioxidants on in vitro development of embryos.