الفهرس 
Aim of the workOnly 14 pages are availabe for public view
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Abstract
The anti-tumor effects of three natural compounds; epigallocatechin-3-gallate (EGCG), resveratrol (RSVL) and docosahexanoic acid (DHA), alone or in combination with cisplatin (CP), were evaluated in Ehrlich ascites carcinoma-solid-tumor bearing mice. Concomitant changes in serum levels of C-reactive protein (CRP), lipid peroxidation (measured as malondialdehyde ”MDA”) and leukocytic count were evaluated following the treatment with these compounds. Further, the possible protective effects of these natural compounds against CP-induced nephrotoxicity in rats were evaluated and the possible mechanisms involved therein. Results: The studied natural compounds elicited significant, dose-dependent reductions in tumor size as well as in leukocytic count, CRP and MDA levels. Their lower doses markedly enhanced the chemopreventive effects of CP. Correlation studies revealed a high degree of positive association between tumor growth and each of CRP (r= 0.89) and leukocytosis (r=0.79).
On the other hand, a single CP dose (10mg/kg) induced nephrotoxicity in rats that was evidenced by increased serum levels of both urea and creatinine. Kidney-homogenates from CP-treated rats displayed significantly-elevated MDA, and -decreased reduced glutathione (GSH) levels. Rats treated with EGCG (50 mg/kg) or DHA (250 mg/kg) survived the lethal effects of CP, and showed a significant recovery of kidney functions; while their homogenates had markedly-reduced MDA, and -increased GSH levels.
Conclusion: These compounds elicited prominent chemopreventive effects on their own, and appreciably augmented those of CP as well. The extent of tumor progression in various mouse groups was highly reflected by CRP levels (thus, implying a diagnostic/prognostic role for CRP). Further, this study is the first to reveal that EGCG and DHA can obliterate the lethal CP-induced nephrotoxicity and renal tissue injury. At the molecular level, they appear to act by reducing inflammation, leukocytosis, oxidative stress, and by replenishing the endogenous anti-oxidant machinery, like GSH.