الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
 |  | from | 108 |  |  |
| | | from | 108 | | |
Abstract
This work was carried out at the Genetics Department, Faculty of
Agriculture, Ain Shams University.
The aim of this work was to Screen and isolate the soil fungal
community from rhizosphere and non-rhizosphere soil from newly
reclaimed lands in comparison with uncultivated ones (desert).
In addition to identifying the obtained fungal spices using ITS
rDNA sequencing. followed by Investigating the effects of soil
physicochemical properties on the fungal communities on the structure,
composition, and metabolic profiles, and determining the strains with the
potential ability to solubilize phosphor and produce indole acidic acid
(IAA) in vitro.
To produce a baseline for the future development of fungi based
on biofertilizers, and biocontrol plans associated with land reclamation.
The research process was as follows:
1- Performing soil physicochemical analysis to determine sample
elements ratios ex; Soil pH, moisture content (MC), total carbon (TC),
total nitrogen (TN), organic carbon (SOC), organic carbon (TOC), Soil
microbial biomass carbon (MBC) and nitrogen (MBN).
Isolation and enumeration of most representative fungal colonies
from the collected soil samples, using final culture media (DRBC, and
PDA).
2- Extract the genomic DNA from each isolated fungal strain, perform
molecular identification using the ITS rDNA gene which was
amplified using a universal primer pair (ITS1 and ITS4), and PCR
products were sequenced.
3- Perform a phylogenetic analysis of the obtained sequences to
determine their taxonomical position, followed by registration in the
gene bank database.
54
SUMMARY
Mostafa N. M. Ali (2023), M. Sc., ALARI., Ain Shams Univ.
4- Testing the obtained fungal strains for their ability to solubilize
phosphor and produce IAA in vitro.
The obtained results were as follows:
Among the screened soil samples 272 fungal strain was counted
and isolated, yielding 27 filamentous fungal species belonging to phyla
Ascomycota, from eight genera. Most of the fungal isolates were
identified at the species level as Aspergillus, Penicillium, Epicoccum,
Fusarium, Ulocladium, Trichoderma, Cladosporium, and Chaetomium.
The 27 fungal strains were subjected to test their ability for P
solubilization, and IAA production with tryptophan in different
concentrations at different pH levels.12 strains were able to produce
different sizes of halo p zone around the colony growth on PVK media,
while 10 species resulted positive and varied in their ability to produce
IAA which proved their potential ability to be used as a microbe-based
phosphatic fertilizer, and plant-promoting growth (IAA) Eco-friendly
biofertilizers.
The findings of the current study can serve as a baseline for the
future development of fungal-based antagonistic on soil microbiome,
plant growth, and biocontrol research. It can provide an outline of the
pathogenic fungal populations associated with the vegetation type of the
studied orchards (i.e., Citrus and Olives). Meanwhile the obtained data
could suggest that colonization of fungal in the rhizosphere, and nonrhizosphere
of the studied locations is independent of the P-solubilizing
activity and IAA production, which could have a prospective effect on the
growth and yield of the plantation, provide potential candidates as
biofertilizers