الفهرس 
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Abstract
est improvArthritis is one of the most important diseases causing lameness in dogs as well as other species of animals. Osteoarthritis (OA) cause considerable deterioration of the cartilage and marked enzymatic changes in the synovial fluid. The present study was conducted on 25 clinically healthy male Mongrel dogs, with body weight of 15.0 ±1.7kg. Arthritis was induced in 20 dogs and 5 dogs were left as a negative control (normal) group. Osteoarthritis was induced by performing arthrotomy and curettage of the cartilage by creation of two 8.0 mm diameter full thicknesschondral defect till exposing the subchondral bone. The dogs were left for 10 days till complete healing of the surgical wound. Amphotericin-B was injected intra-articularly at a dose of 10 mg weekly for 2 successive weeks. The dogs were left one week till complete typical symptoms of arthritis were observed. The experimental groups were classified into five groups as control negative (normal), dexamethasone treated, sodium hyaluronate treated, poly sulfated glycosaminoglycan treated and control positive (sham operated). Sensory characteristics of the treated groups were evaluated. Besides, synovial analysis was performed for each animal at the end of the 1st, 2nd, 3rd, 4th weeks. Matrix metalloproteinase (MMPs) 3 and 13 and tissue inhibitor metalloproteinase (TIMP-1) were detected by ELISA assay. The obtained results revealed that osteoarthritis OA was associated with significant increases in the enzymatic activities of MMPs 3 and 13 and decrease of TIMP-1 as biomarkers of OA. Among the used treatments, polysulfated glucose aminoglycan is the highly recommended therapeutic approach as it caused the highement compared with other treatments.
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