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Abstract
In the present study 42 staphylococcus isolates from 220 raw bovine milk samples (150 from buffaloes and 70 from cows) were collected from different farms, samples from buffaloes were identified as 35 normal milk samples, 90subclinicallymastitic milk samples and 25 clinically mastitic milk samples, while the total 70 cow{u2019}s milk samples were identified as 45 normal milk samples, 10 subclinicallymastitic milk samples and 15 clinically mastitic milk samples. the total Staphylococcus species isolates were 42(19%) from 220 raw milk samples(27 from buffaloes and 15 from cows) 66.6% from the total isolates were originated from subclinicallymastitic animals, 28.6% from healthy animals and 4.8 from clinically mastitic animals,Bacterial identification indicated that CNS constituted the higher percentage 71.4%(30 isolate), while CPS isolates constituted 28.6% from the total isolates(12 isolates)milk samples from cows and buffaloes were coagulase positive in a percentage of 28.6% and 71.4% for coagulase negative Staphylococci. The highest prevalence of isolation in the CPS identified as S. aureus in a percentage of 26.2% 11 isolate and lower for S. intermedius 1 2.4%