الفهرس 
IntroductionOnly 14 pages are availabe for public view
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Abstract
In this work, gelatin was modified by two monomers, namely, methacrylic anhydride (MAA) and glycidyl-methacrylate (GMA) to obtain photo-curable macromers. These macromers were then irradiated by UV in order to obtain hydrogels which were taken as biomaterial matrices for graphene derivatives composites. The latter were evaluated in terms of UV-visible spectroscopy, XRD, EDX, SEM, and TEM. Furthermore, these composites were assessed in three medical fields, explicitly, burn wound management; anticancer and blood contacting biomaterials.
Our results demonstrated that the composite hydrogel (Gel-MA/rGO) show high antibacterial activities than the virgin (Gel-MA). Furthermore, the incorporation of Ag-NPs enhanced the role of rGO to 120% and 104% for S. aureus and P. vulgaris, respectively. Moreover, it was observed that the curcumin (as an anti-inflammatory drug) loaded hydrogels have the cumulative percent release values of 80% & 50% for Gel-MA/rGO and 70% & 43% for Gel-MA/rGO/Ag nano-composite within 24 h at pH 7.4 and 5.4, respectively. These results indicated the slower release of drug in silver nano-composite hydrogel. Additionally, the cytotoxicity of the prepared Gel-MA/rGO and Gel-MA/rGO/Ag composites against human lung cancer cells (A549) was evaluated. The latter one showed remarkable cytotoxicity with IC50 of 27µg/ml compared with 109µg/ml of the first one.
With respect to blood contacting biomaterials, the prepared hydrogel composites (Gel-GMA & Gel-GMA/GO) had optimistic hemolysis ratio (less than 5%); international normalized ratio =1; slow rate of degradation and negligible cytotoxicity on healthy human lung fibroblast cells (MRC-5); which may make them the milestone for a variety of short-term blood conducting materials.