الفهرس 
Circulating tumor cells and microsatellite instability in colorectal cancer
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Abstract
Background Microsatellite instability (MSI) is an important piece of information to consider when deciding whether to use adjuvant chemotherapy in patients with stage II colorectal cancer (CRC), it{u2019}s a marker of a more favorable outcome and a predictor of decreased benefit from adjuvant Fluoropyrimidine containing chemotherapy in stage II CRC. Circulating tumor cells (CTC) has shown prognostic and therapeutic implications in breast, colon and prostate cancer. CTC detection by CellSearch System and other sensitive techniques is an easy and highly reproducible method that correlates mostly with tumor stage. Methods This was a retrospective study that included all CRC patients diagnosed and received treatment at NCI Cairo that underwent evaluation of MSI & CTC levels, in the period between 2012 till 2013. MSI assessment was performed by obtaining the paraffin-embedded tissue sections of the tumors from the pathology department, DNA was extracted and amplified using the MSI analysis system which is a fluorescent PCR-based assay. CTC enumeration and identification was done through flow-cytometry and PCR. Collection of blood samples was done before and during treatment. MSI status and CTC level were correlated with the clinical features of the patients, response to treatment and survival