الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 177 |  |  |
| | | from | 177 | | |
Abstract
Background: Successful implantation requires an intricate succession of molecular and genetic interactions. Therefore, this process is controlled by a number of factors like ovarian hormones, cytokines and growth factors which play an important role in regulating embryo differentiation, invasion and adhesion. During this time any change in the communication between the endometrium and the embryo leads to implantation failure. Activin A hormone and Betacellulin (BTC) growth Factor have a positive effect upon cell number, implantation and human blastocyst development. Fibrillin- 3 is more recently discovered hormone and thought to be primarily present during development of follicles. Unfortunately, although the huge development in ART, pregnancy and live birth not exceed the percentage 30-40% in best IVF center. Aim of the study The aim of this study was to evaluate the main factor(s) that responsible for the implantation failure after ART program that included: Measure the levels of Activin-A and Fibrillin-3 hormones and Betacellulin growth factor at different phases of the menstrual cycle in a controlled ovarian stimulation program.To elucidate the correlation between the levels of these hormones and betacellulin growth factor with successful or failure of implantation following IVF /ICSI cycles. Subjects , Materials and methods: The study was designed as prospective study. The sample number was 100 subfertile women undergoing intracytoplasmic sperm injection (ICSI). The selected 100 women were intentionally divided, according to the cause of infertility, into 50 infertile women with polycystic ovary syndrome (PCOS) and 50 infertile women without PCOS (male factor). Samples collection were done at Kamal Al-Samara’ay IVF center-Ministry of Health in Baghdad-Iraq. The duration of the study extended from September 2020 to June 2021.Full history, detailed physical examination and investigation were applied to all women participating in the current study. IVF was done according to predefined protocols and the outcome of pregnancy was then follow up with all variables included in the current study. Results: Mean age of non-PCOs and PCOs women was (30.44± 3.89 versus 31.16± 2.41 years) respectively and there was no significant difference in the mean age between non-PCOS group and PCOS group. The number and percentage of women having primary infertility were less in the group of non-PCOS than in the group of PCOS, 27 (45%) versus 33 (55%), respectively, the difference was not statistically significant (P=0.4386).On the other hand, number and percentage of women complaining of secondary infertility were more in the non-PCOS group than in the PCOS group, 23 (57.5%) versus 17 (42.5%), respectively; however, the difference was not statistically significant (P=0.3428). The results showed highly significant differences were encountered between non- PCOS and PCOS groups regarding serum and follicular fluid of activin A, fibrillin -3 hormones and betacelullin growth factor at day of ova pick up and embryo transfer day. The level of activin- A hormone was highly significant in PCOS group compare to non- PCOS group, while the level of fibrillin -3 hormone and betacelullin growth factor were highly significant in non-PCOS group compare to the PCOS group at day of ova pick up and embryo transfer day. Results of real time PCR quantification of ACT A gene expression: - The fold of gene expression in non-PCOS group decreased by (0.85) compared to the PCOS group at the dayof ova pick up.