الفهرس 
IntroductionOnly 14 pages are availabe for public view
 |  | from | 103 |  |  |
| | | from | 103 | | |
Abstract
One of the most important reasons for endodontic treatment failure is the persistence or survival of microorganisms in the complex root canal system or periapical area; therefore, the success of endodontic treatment depends, to a great degree, on the elimination of microorganisms from the root canal system through mechanical instrumentation and chemical irrigation. Profound irrigation of the canals with antimicrobial solutions is an important step to eliminate or decrease the number of microorganisms from the root canal system. Enterococuss faecalis (E.faecalis) has been reported in high prevalence in primary endodontic infections affecting children.
The purpose of the current study was to compare the antimicrobial effect of 20% ginger ethanolic extract solution with 2% chlorhexidine when used as root canal irrigants in extracted primary teeth contaminated with E. faecalis bacteria.
Seventy-five extracted deciduous maxillary teeth were de-coronated
.Then, the roots were separated and only palatal roots of molars teeth were used. Roots were randomly divided into three experimental groups, one positive control group and one negative control group as follows: group I (negative control): consisted of 15 roots that were not contaminated nor irrigated,group II (positive control): consisted of 15 roots that were contaminated and irrigated with sterile saline, group III: consisted of 15 roots that were contaminated with E.faecalis and irrigated with 20% ginger solution,group IV: consisted of 15 roots that were irrigated with 2% Chlorhexidine solution after being contaminated with E.faecalis and GroupV:consisted of 15 roots that were irrigated with 95% ethanol after being contaminated with E.faecalis.
The ginger solution was prepared through dissolving in pure ethanol (95%) to bring about a concentration of 2-gram powder/10 ml to give a 20% ginger solution that was used as irrigation material.
After mechanical preparation of the root canals employing the step back preparation technique reaching a master apical file size #40 to standardize the diameter of all the canals, sterilization of all samples was carried out using Andromeda vacuum xp autoclave at 121- degree °C and 15 PSI pressure for 15 minutes.
Bacterial contamination groups (II,III,IV,V) with E. feacalis were carried out by immersing the samples in a 24- hour pure culture suspension of E.faecalis grown in Brain Heart Infusion (BHI) broth and adjusted to No. 1 MacFarland turbidity standard. All the roots were incubated at 37 °C in sealed vials. This procedure was repeated every 72 hours using a 24-hour pure culture prepared and adjusted to the No.1 MacFarland turbidity standard.
Roots in each group were irrigated with their assigned irrigation solution and kept in the canal for 5 minutes then samples were collected from the canals using two sterile paper points from each sample.
Results showed that the highest antibacterial activity of the irrigants was observed for CHX followed by 95% ethanol then 20% ginger ethanolic extract solution.While statistically there was no significant difference between ginger ethanolic extract solution and 95% ethanol.
Within the limitation of this study, it was conclouded that adding ginger in 20% concentration to 95% ethanol did not add to the antibacterial effect of ethanol.