الفهرس 
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Abstract
Psoriasis is a multifactorial disease in which some environmental factors acting on people who have a particular genetic predisposition trigger immune dysregulation and irregular keratinization, resulting in typical cutaneous lesions. It is a genetically based chronic inflammatory skin condition characterised by epidermal hyperproliferation and irregular keratinocyte differentiation, T-lymphocyte infiltration, and increased expression of cytokines, which results in the formation of inflamed plaques. The interaction of the gut microbiome, intestinal barrier, and immune system has recently attracted a lot of attention. Tight junctions bind epithelial cells in the gastrointestinal epithelial barrier, which is a complex network of epithelial cells. Inflammatory and metabolic comorbidities of psoriasis, such as arthritis and inflammatory bowel disease, have been related to disturbances in the intestinal barrier. Claudins are transmembrane proteins that bind to the actin cytoskeleton and aid in the formation of tight junctions. The loss of gut barrier function occurs when unique claudins are knocked out. The presence of Claudin3 in the blood is thought to be a useful biomarker of intestinal permeability. The aim of this study was to compare the serum levels of Claudin-3 in patients with psoriasis to those in a control group, and to see if the levels were linked to the severity of the disease. This study included 53 psoriasis patients (32 males and 21 females) and forty healthy controls (23 males and 17 females) who were age and sex matched to the cases category. They chose at random from the outpatient clinic of Mansoura University Hospital’s Dermatology Department. Typical clinical results were used to diagnose the patients. The following data were calculated for all patients and the control group: age, sex, BMI, family history, BP, history of smoking, and disease duration for all patients and the control group. The magnitude of psoriasis was also assessed using the PASI score. All patients were undergone laboratory test for their lipid profile (TGs, LDL, HDL, Cholesterol) and serum level of claudin -3: 5ml morning venous blood samples were collected from each person included in this study after an overnight 12‐h fast. Blood was centrifuged, and sera were immediately separated and stored at −80 °C for further analysis. Assessment of serum claudin -3 was done by an ELISA technique. Statistical kit for Social Science (IBM Corp. Released 2017) was used to rewrite, code, tabulate, and upload the collected data to a computer. Version 25.0 of IBM SPSS Statistics for Windows (Armonk, NY: IBM Corp.). Data was provided, and sufficient analysis was performed for each parameter based on the type of data collected. Results of the study : * Psoriasis cases were significantly associated with higher frequency of positive family history and smoking (p=0.048, 0.001 respectively). * Smoking was significantly associated with higher Claudin-3 level (p=0.031). * In addition, Claudin-3 level increased gradually with increased severity grades (p<0.001). * No significant associations were found regarding Claudin-3 level according to gender, nutritional status, family history, in Psoriasis group (p>0.05 for each). * Positive family history, smoking and higher claudin-3 level were significantly associated with prediction of psoriasis development in univariable analysis, When significant covariates from the univariable analysis were added to the multivariable analysis, only a higher Claudin-3 level was found to be an independent predictor of psoriasis growth. * Higher BMI, smoking and higher Claudin-3 level were associated with prediction of higher PASI score in univariable analysis. While multivariable analysis revealed that only smoking and higher Claudin-3 level were considered independent predictor of more severe psoriasis cases.