الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Irradiation induced depolymerization of chitosan
solution through scission in - (1-4)-glycoside bond resulted
in decreasing the chain length and reduction in molecular
weight. By increasing the irradiation dose, the solubility of
chitosan increases and the viscosity shows a decreasing
trend. NOC was successfully prepared by -irradiation at 30
kGy under appropriate physical formulations of chitosan and
irradiation doses, which is confirmed by the characterization
of chitosan with measuring the relative viscosity and
scavenging activity. NOC is considered an enhanced form of
the normal chitosan where it gathers the effective
characteristics of chitosan such as antioxidants effect, in
which the active groups OH and C=O, which are sites for
interaction and modification increased, besides the unique
properties of the nanoparticles such as small size.
Pretreatment of rats with NOC followed by exposure to -
irradiation, CCl4 intoxication, or both treatments ameliorated
the induced hepatoxicity.The current study aimed at investigating the
hepatoprotective potential of NOC against -irradiation
and/or CCl4-induced liver injury in male rats.
The animals were randomly divided into 8 groups:
1) A negative control group
2) NOC group
3) γ-Irradiated group
4) NOC + -irradiated group
5) CCl4 group
6) NOC + CCl4 group
7) CCl4 + -irradiated group
8) NOC + CCl4 + -irradiated group
The determination of relative viscosity of NOC.
The determination of scavenging activity (%) of NOC.
characterization of NOC.
FT-IR spectroscopy.
UV-Vis Spectroscopy.
DLS.
TEM.
Liver function tests (ALT, AST, ALP, GGT and LDH
activities as well as bilirubin concentration) were assessed
in the serum.
Protein profile (T.PRO, ALB, GLO and A/G ratio) was
assessed in the serum. MDA and PCC levels were determined in the liver tissue
homogenate.
The mRNA expressions of NFB, IL-1, Bax and Bcl2, as
well as Bax/Bcl-2 ratio were determined in the liver tissue
homogenate.
Histological examination of the liver.