الفهرس 
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Abstract
The present study was directed to investigate the prevalence of brucellosis in the milk of small ruminants in terms of evaluation of the commonly used diagnostic bacteriological, molecular and serological procedures as well as identification and typing of the causative Brucella species in small ruminants. So blood serum samples and milk samples of 610 animals from 7 herds of sheep from Mahfoza village- Bilqase town- Dakahlia Governorate were examined for brucellosis by using BAPAT, RBPT and MRT and the results showed that, 48 (7.8%), 44 (7.2%) and 41 (6.7%) respectively. In the current study, an isolation rate of 29.6% was observed. Sixteen Brucella strains were isolated from all examined 3/3 aborted fetuses, from 1/10 tissue specimens of ten slaughtered serologically positive ewes and 12/41 milk samples. The result obtained in this study showed that all the field isolates of B.melitensis were typed as biovar 3. In this study, evaluation of the test and slaughter program for control ovine brucellosis was investigated. To evaluate the test and slaughter strategy in two herds, herd numbers 2 and 4, we used BAPAT and MRT as serological tools to eliminate the positive reactors, respectively. The results showed that the periodical testing of sheep with rapid elimination of positive cases could virtually eliminate the source of infection among sheep herds through the slaughtering of positive reactors that resulted in a gradual decrease of prevalence of reactors till it reached (zero%) beginning of the 8th examination up to 12th examination using BAPAT. Evaluation of test and slaughter program in sheep depends on MRT results in herd 4, proved to us that we cannot depend on MRT alone for diagnosis of positive animals. In the current study, AMOS PCR was a perfect method for rapid, sensitive, and accurate Brucella detection at the species level. AMOS PCR identified three isolates as B. melitensis.