الفهرس 
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Abstract
The present study was designed to study the prevalence and characteristics of pathogenic E.coli strains from sheep and goats. In this study, 170 samples were collected from sheep, goats and bedding (136, 27 and 7) respectively. Samples were collected as 134 fecal samples (34 diarrheic and 100 apparently healthy sheep and goats), 29 milk samples (25 sheep milk and 4 goat milk) and 7 samples from bedding (5 sheep and 2 goats ) respectively. The samples were collected from different sheep and goat farms at Badr City in Al Beheira Governorate, Egypt.
E.coli was isolated from sheep and goats with a percentage of 44% and 31% respectively. Serogrouping of E.coli isolates from sheep showed 7 different types ”O” serogroups; O26, O111, O55, O125, O86, O121, O103. While in goats showed that they were belonged to 2 serotypes; O111, O124. E. coli isolates were screened by multiplex PCR assay for the presence of virulence genes characteristic for E.coli (stx1, stx2, eaeA and hlyA).
Of the 12 E.coli isolates from sheep, 11 (91.7%) isolates carried the stx1 genes and 12(100%) isolates carried the stx2 genes. EaeA genes were found in 5 (41.7%) isolates and 8 (66.7%) isolates were positive for hlyA genes. In goats from 3 E.coli isolates by PCR showed that 2 (66.7%) isolates carried stx1, stx2 and hlyA genes. While the eaeA gene was detected in 3 (100%) isolates.
The resistance of 15 isolates was observed most frequently to Ampicillin (66.7%), Trimethoprim/ Sulphamethoxazole (73.3%), Cephotaxime (86.7%), Amikacin (60%), Gentamicin (166.7%), Nalidixic acid (53.4%), Pencillin (60%), Ciprofloxacin (53.4%), Streptomycin (53.4%) and Norocillin (46.6%) while less frequently to Chloramphenicol (13.3%), Doxycilline (20%), Amoxicillin /Clavulonic acid (46.6%) and Cephrodine (26.4%).