Search In this Thesis
   Search In this Thesis  
العنوان
Prevalence, molecular characterization and antimicrobial resistance profile Of salmonella in retail chicken meat /
المؤلف
El-Sayed, Hagar Hamed Mahmoud Ramadan.
هيئة الاعداد
باحث / هاجر حامد محمود رمضان السيد
مشرف / خالد ابراهيم سلام
مشرف / محمود احمد محروس
مشرف / حازم حسن رمضان
الموضوع
Salmonella. Poultry meat.
تاريخ النشر
2020.
عدد الصفحات
online resource (117 pages) :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
Veterinary (miscellaneous)
الناشر
تاريخ الإجازة
01/7/2020
مكان الإجازة
جامعة المنصورة - كلية الطب البيطرى - الرقابة الصحية على الاغذية
الفهرس
Only 14 pages are availabe for public view

from 117

from 117

Abstract

This study aimed. to determine the prevalence and genetic characterization of salmonella isolated from 100 local frozen chicken carcasses purchased from supermarkets shops distributed at Mansoura city, Egypt, that were subjected for both phenotypic and genotypic analyses. The present study was conducted on 100 chicken carcasses. from one hundred carcasses there were 76 identified salmonella isolates were further verified using PCR tool for the presence of a species-specific gene (invA gene) which appeared highly conserved in Salmonella isolates. (all 76 isolates were salmonella). Serological identification was done which revealed that there were 12 serotypes of salmonella. S.Typhimurium was (23.68% n=18) , S.Enteritidis (18.42% n=14) , S.Kentucky (15.78% n=12) , S.Molade (10.52% n=8) ,S.Tsevie (7.89% n=6) ,S.Anatum (5.26% n=4) , S.Tamale (5.26% n=4) S.Takoradi (2,63% n=2) , S.Larochelle (2.63% n=2) , S.Infantis (2.63% n=2) , S.Heidelbrg (2.63% n=2) , untypable (2.63% n=2) .Antimicrobial susceptibility test was done on 76 molecular confirmed Salmonella isolates by using minimum inhibitory concentration method (MIC) against 14 different antibiotics which revealed that, the predominant resistant against Erythromycin was 100%, followed by 94.7% of isolates were resistant against Oxacillin, then 86.8% were resistant against penicillin, 76.3% were resistant against cephalothin, 63.2% were resistant against cefotaxime, 46.4% While lower resistance was against Gentamicin (2.6%).