الفهرس 
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Abstract
The present study was designed to determine the effect of different concentrations of cadmium chloride and lead chloride and their mixtures on some haematological and biochemical parameters in one hundred and sixty-eight of healthy catfish Clarias gariepinus after calculating the lethal dose of half of one hundred and sixty fish of the same fish collected from Lake Manzala, (Egypt), they were fed and acclimatized for two weeks before the start of the experiment for sixty days. The fishes were divided into seven groups; 8 fishes in each. All treatments were run in triplicates as follows: Control Group: this group only was fed, left without any treatment, meanwhile second and third group with different cadmium concentration (Cadmium (10%& 20%, respectively). Others, fifth and sixth with lead with different concentration (Lead (10%& 30%, respectively). Finally, two groups with mixture of Cd and Pb with two concentration (10%& 30%).the results clarified that reduction in Haemoglobin (Hb), thrombocytes count, mean corpuscles volume (MCV) with increasing the number of Eosinophil cell were concluded at the groups treated with cadmium. On the other hand, decreasing the activity of Glutathione level (GSH) in fish treated with Cd at10% & 30% in GSH. On opposite to the activity of Catalase (CAT), malondialdehyde level (MDA), and Superoxide dismutase was significantly high at fish treated with Cd (30%, CAT & 10% and 30% for two last enzymes). Plasma sodium and total protein of fish treated with Cd 10% of 96 hrs LC50 value were significantly low. While the plasma potassium level of fish treated with Cd10% and Cd 30% of 96 hrs LC50 value was significantly low. Aspartate aminotransferase activity (AST), the plasma ammonia, uric acid, bilirubin and creatinine levels of fish treated with Cd10% and Cd 30% of 96 hrs LC50 value were significantly high, while alanine aminotransferase (ALT) activity and cortisol level of fish treated with Cd 30% group of 96 hrs LC50 value were significantly high.Plasma total lipids and cholesterol levels of fish treated with Cd10% and Cd 30% of 96 hrs LC50 value were significantly low, while triglycerides level were significantly lower in Cd10% of 96 hrs LC50 value were significantly low.
The groups that treated with different concentration and revealed that low count of Hb of fish treated with Pb10% of and thrombocytes count of fish treated with Pb30% was significantly low.Glutathione level (GSH) of fish treated with Pb10% was significantly low.High activity of CAT, malondialdehyde level, and SOD of fish treated with in Pb30% of 96 hrs LC50 value was significantly high. Plasma sodium and Plasma cholesterol of fish treated with Pb10% of 96 hrs LC50 value was significantly low. While, plasma ammonia, uric acid, creatinine and cortisol of fish treated with Pb10% and Pb30% of 96 hrs LC50 value were significantly high. The group of mixture of cadmium and lead clarified high count of Mean corpuscles volume (MCV) and the mean corpuscles haemoglobin (MCH) and thrombocytes count of fish treated with mix 10% and 30%. On the other hand, high activity of Catalase activity (CAT), malondialdehyde level, and Superoxide dismutase of fish treated with (mix10% and 30%) in of 96 hrs LC50 value % was significantly detected. Plasma sodium and total protein and cholesterol levels of fish treated with (mix10% and 30%) of 96 hrs LC50 value were significantly low. On the other hand, Plasma ammonia and bilirubin levels of fish treated with (mix10% and 30%) were significantly high. While the uric acid and triglyceride levels of fish treated with in mix 10% were significantly high. In addition, ALT enzyme and creatinine level of fish treated with mix 30% were significantly high.