الفهرس 
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Abstract
The aim of the current study was to evaluate serological tests in comparison with real time PCR for diagnosis of brucellosis in sheep and goat. Therefore, six hundreds blood samples were collected from apparently healthy and brucellosis suspected sheep and goats brought to the Veterinary Clinical Complex from areas in and around Al Bida, Al Jabal Al Akhdar, Libya. Blood samples were subjected to serological tests including Rose Bengal Plate test (RBPT) and Enzyme-Linked Immunosorbent Assay (ELISA) for detection of IgA and IgM. Serologically examined samples were also subjected for real time PCR for detection of Brucella DNA. Out of the total examined samples 45 (7.5%), 39 (6.5%), 3(0.5%) and 49 (8.16%) were tested positive by RBPT, ELISA IgG, IgM and real- time PCR respectively. Sensitivity and specificity of RBPT compared with real- time PCR were 79.59% and 98.9% respectively. While, sensitivity and specificity of ELISA for IgG with real time PCR were 79.59% and 100% respectively, while, for ELISA IgM were 6.12% and 100% respectively. Real time PCR versus ELISA IgG, IgM together the ELISA assay have a sensitivity of 85.7% {42/(42+7) 100} and a specificity of 100% {551/(551+0 )100}. So, the PPV and NPV of ELISA IgG, IgM are 100% {42/(42+0)100} and 89.7% {551/(551+7)100} respectively In conclusion, we suggest using real- time PCR as a supplementary test in the diagnosis of brucellosis and as a confirmatory test in suspicious cases.