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العنوان
Studies on Guava Propagation by Tissue Culture /
الناشر
Ahmed Abd El Samee Mustafa Attia،
المؤلف
.Attia, Ahmed Abd El Samee Mustafa
هيئة الاعداد
باحث / Ahmed Abd El Samee Mustafa Attia
مشرف / F. A. El-Dengawy
مشرف / M. Abu Elyazid
مناقش / Farouk AbdEl-Hamied El-Morshdy
الموضوع
guava. propagation.
تاريخ النشر
2018.
عدد الصفحات
108 ص. :
اللغة
الإنجليزية
الدرجة
ماجستير
التخصص
البساتين
تاريخ الإجازة
26/1/2018
مكان الإجازة
جامعة دمياط - كلية الزراعة - الفاكهة
الفهرس
Only 14 pages are availabe for public view

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from 139

Abstract

from the obtained results it could be concluded that guava nodal explant could be propagated in vitro through this protocol, surface sterilization by immersion in clorox 40 % for 15 min. the best antioxidant treatments was as following:
1- Using the second node of shoot tip.
2- Collecting explants in spring season with culturing in vertical position.
3- Sealing cut ends of collected explants before culturing with molten paraffin wax.
4- Pretreatment of explants with citric acid at 100 mg /l, 150 mg /l ascorbic acid and 0.5 % PVP.
5- Adding adsorptive or antioxidant material to ms medium (citric acid at 100 mg /l +150 mg /l ascorbic acid).
6- Incubation of cultured explants on darkness for 7 days.
7- Incubation explants after culturing on 4 º C for 7 days.
The MS media supplied with 1.0, 1.5 mg /l BA gave the highest average number of shoots and Adding (1.0 mg /l BA + 0.2 mg /l IBA) and (1.5 mg /l BA + 0.5 mg /l IBA) to MS media gave the best effect on average length of explant and average number of leaves. Addition of 1.5 mg /l GA3 to half strength MS media gave the average length of explants. The best rooting of shoots was in MS medium at half strength with 1.0 mg /l IBA and the rooted plants were acclamized successfully.
These treatments are recommended to guava propagation by tissue culture techniques.