الفهرس 
INTRODUCTIONOnly 14 pages are availabe for public view
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Abstract
Fluconazole short half-life in the eye results in frequent administration and poor patient compliance.
Our goal was to prepare and optimize fluconazole loaded gel core liposomes to increase the ocular residence time and corneal permeation of fluconazole. In this thesis, gel core liposomes were formulated and appraised using two different polymers namely; hyaluronic acid (HA) and carbopol (CB).
The work in this thesis was divided into two Chapter One: Development and In-vitro Appraisal of fluconazole-loaded ophthalmic gel-core liposomes using two different polymers: hyaluronic acid and carbopol Fluconazole loaded gel-core liposomes were prepared by spontaneous thin film hydration method.
Different factors including; the type and concentration of the hyaluronic acid (hyaluosomes) and carbopol (carbosomes), the additives in the bilayer structure, and initial drug concentration were optimized. The optimum gel core liposomes were selected based on the perfect gel-core structure, entrapment efficiency, particle size and stability of the vesicles. The structure of prepared gel-core liposomes was studied using optical, polarizing microscopes and transmission electron microscope (TEM). The conformation of gel formation in the core was confirmed by triton-X addition. The spherical structure of the vesicles was retained even after the bilayer removal. The %EE of fluconazole was increased in the prepared gel-core liposomes by increasing the initial drug concentration.
The amount of FLZ entrapped in gel-core liposomes formulated using hyaluronic acid (HYS2-HYS8) was increased from 0.77 mg/ml ± 0.009in HYS2 (0.3% w/v FLZ) to 11.73mg/ml ± 0.12 in HYS8 (1.2% w/v FLZ). Carbopol-based gel-core liposomes (CBS1-CBS6) showed increased amount of FLZ entrapped from 0.97mg/ml ± 0.02 in CBS1 (0.3% w/v FLZ) to 6.89mg/ml ± 0.33 in CBS5 (1.2% w/v FLZ). The particle size of selected gel-core liposomal formulations (HYS7, HYS8, and CBS5) was 218.5, 320, and 339 nm respectively and zeta potential of selected fluconazole gelcore liposomes HYS7 (HA 0.7% and FLZ 0.9%) and CBS5 (CB 0.3% and FLZ 0.9%) were found to be -42.8 mV and -41.7 mV. Additionally, the in vitro release profiles were investigated and compared to the FLZ suspension, FLZ in HA gel, FLZ in CB gel and conventional liposomes (LS2). The selected gel core formulations (HYS7 and CBS5) showed sustained release.