الفهرس 
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Abstract
Background: Salivary gland stem cell therapy is an
attractive putative option to treat various salivary gland disorders
and cases causing salivary hypofunction.
Aim: The aim of this work is to isolate stem cells from the
Submandibular and Parotid salivary glands of albino rats for
identification and characterization. Also, to assess the
proliferation rate, the cryopreservation ability, and to culture
isolated stem cells in cell culture inserts.
Methodology: Fifteen adult male albino rats were used in
this study. The Submandibular and Parotid salivary glands were
dissected and prepared for tissue culture. Identification and
characterization were carried out through assessment of
proliferation rate, performing flow cytometry analysis and colony
forming unit-fibroblast assay. Cryopreservation, Culturing of stem
cells in cell culture inserts and ultrastructural study of the SG stem
cells using Scanning electron microscopy were also carried out.
Results: Stem cells from both glands were successfully
isolated and were positive for salivary gland stem cell markers
CD133 and CD117. The Submandibular salivary gland stem cells
showed faster and higher proliferation rate and also formed more
colonies than the parotid gland stem cells. Stem cells from both
glands formed multicellular layers when cultured on the
transmembrane culture inserts yet the submandibular multicellular
layer was apparently thicker than that of the Parotid gland.
Cryopreservation was performed for both groups and thawed cells
of the Submandibular group showed a higher percentage of
viability than the parotid group.
Conclusions/Significance: Rats salivary glands contain a
‘putative’ stem cell population, expressing salivary gland stem
cell markers that are capable of forming multicellular layers when
cultured on transmembrane cell culture inserts. Also, stem cells
isolated from salivary glands can be successfully cryopreserved
showing sufficiently high number of viable cells after thawing.