الفهرس 
Introduction and Aim of WorkOnly 14 pages are availabe for public view
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Abstract
Blood transfusion can be a life-saving procedure, but it has risks, including infectious and nonin¬fectious complications.
Parvovirus B19 (PVB19) is one of the emerging transfusion transmissible infections. Infections with parvovirus B19 are quite common, particularly in certain high-risk population.
The major high-risk groups are children,pregnant women, patients with underlying haematological problems and immune-deficient patients who constantly are multi-transfused. The pattern of clinical disease caused by B19V varies and is influenced by both the hematological and the immunological status of the infected individual.
Human erythrovirus (parvovirus) B19 causes a wide range of diseases, such as erythema infectiosum or fifth disease,a common illness in children, aplastic crisis, chronic pure redcell aplasia, fetal hydrops and fetal death. The virus is associated with arthropathies, hepatitis and various other syndromes and diseases.
Current attempts, using sensitive screening tests, to improvise blood safety focus entirely on eliminating the risk of transmitting infectious agents. But yet donor screening is not mandatory.
The aim of our study is to detect prevalence of parvovirus B19 in the donated blood units from healthy donors in Beni-Suef university blood bank and received by a group of high risk recipients i.e. multitransfused children in a trial to reveal the significance of its screening in packed RBCs units and whether these recipients are at risk for developing clinically significant Parvovirus B19 infection after receiving these blood units.
A total of 91 samples of donated blood units from 91 healthy donors in Beni-Seuf University Hospital Blood Bank .Samples were collected in 6 months period from January 2016 till July 2016 and screened for IgM antibodies in human serum aganist Parvovirus B19 by ELISA test .
Our study includes also 91 recipients of these donated blood units, all of them have hematological disorder and are receiving medical care at Pediatric Department of Beni-Seuf University Hospital .
IgM assay was determined using a solid phase enzyme – linked immunosorbent assay (ELISA) kit for IgM anti-PVB19 antibodies. Among our 91 donated units, we found that 8 (8.8%) had a positive Parvovirus B19 IgM antibodies ,while 67 (73.6%) were negative and 16 (17.6%) were at the grey zone.
For each recipient data were collected retrospectively from the medical records for post-transfusion follow up of B19 infections manifestations within one month .
Post-transfusion follow up of the recipients was non-significant and no one of them acquired clinical manifestations of Parvovirus B19 infection.