الفهرس 
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Abstract
This study was performed to detect the possible effects of diazinon on biochemical, histopathological and gene expression of Oreochromis niloticus. It is carried out on one hundred and fifty apparently healthy Oreochromis niloticus fish. Experimental fish were randomly distributed to five groups. The first group (G1): It was kept as a control group in which fish were supplemented with water free from diazinon. Fish in the second group (G2): were supplemented with water treated with diazinon 15% at dose 1/10 of the LC50 of diazinon for 1 week. Fish in the third group (G3): were supplemented with water treated with diazinon 15% at dose 1/10 of the LC50 of diazinon for 2 weeks. Fish in the fourth group (G4): were supplemented with water treated with diazinon 15% at dose 1/10 of the LC50 of diazinon for 3 weeks. Fish in the fifth group (G5): were supplemented with water treated with diazinon 15% at dose 1/10 of the LC50 of diazinon for 4 weeks. This study shows that the chronic exposure to sub-lethal concentration of diazinon induces production of free radicals and oxidative stress that can be indicated by biochemical and histopathological alterations in Oreochromis niloticus and it was observed that diazinon addition can negatively affect glutathione S-transferase gene expression and this offers a simply tool to evaluate diazinon-derived toxicity by monitoring the activities of cellular detoxification agents such as glutathione S-transferase which can be effectively used as biomarkers of pesticide toxicity.