Search In this Thesis
   Search In this Thesis  
العنوان
Biochemical Mechanisms of Insecticide Resistance in
the House Fly Musca domestica Linnaeus
(Diptera: Muscidae)
from certain Governorates in Egypt\
المؤلف
Osman, Dina Abdel Sadek.
هيئة الاعداد
باحث / Dina Abdel Sadek Osman
مشرف / Adel Ramzy Fahmy
مشرف / Mohammed Saad Hamed
مناقش / Azza Ismail Mohamed
مناقش / Azza Abdel Fattah Mostafa
تاريخ النشر
2014.
عدد الصفحات
198p. :
اللغة
الإنجليزية
الدرجة
الدكتوراه
التخصص
علوم الحشرات
تاريخ الإجازة
1/1/2014
مكان الإجازة
جامعة عين شمس - كلية العلوم - علم الحشرات
الفهرس
Only 14 pages are availabe for public view

from 198

from 198

Abstract

SUMMARY
The present study aimed to throw some light on the
insecticidal and biochemical activities resulted from the effect of
some used insecticides in controlling the house fly M. domestica
in the field.
The obtained results are summarized as followes:
Evaluation of different insecticides against adult stage of M.
domestica
1- The susceptibility of populations of three Governorates to
insecticides based on LD50values revealed that toxicity of the
tested compound varied remarkably with insecticides group
comparing with laboratory strain, for two successive years.
2- Screening of pyrethroid insecticide compound deltamethrin
indicated that it was the most effective one among the different
insecticides used to control adult of house fly.
3- Pyrethroids still have the potency to control the pests,
deltamethrin was more effective than malation from
organophosphorus compounds and bendiocarb from carbamate
compounds.
131
􀀉 Summary
4- Bendiocarb from carbamate compounds was more effective
than malathion from OP,s compounds in Cairo, Fayoum and
Ismailia.
5- It was observed that Ismailia population was the most resistant
one among all tested populations followed by Fayoum population
while Cairo population was the most sensitive one.
6- At 2008, it was noticed that some of the tested insecticides had
low LD50, s than which at 2009.
Inheritance mode of resistance to deltamethrin in the house
fly Musca domestica L.:
This work detected the specific mechanisms involved in
resistance to synthetic insecticides commonly used in fly control
program in Egypt.
The mechanisms of inheritance of resistance factor were
studied for Ismailia population that showed high resistance for
these insecticides.
The R strain which collected from Ismailia Governorate and
showed the highest resistant ratio, had the LD50 higher than
F1(S♀×R♂) and F1
-(S♂×R♀) .
132
􀀉 Summary
These results suggested that deltamethrin resistance in
Musca domestica was expressed as an incomplete dominance
trait in F1(S♀×R♂) and an incomplete recessiveness trait in F1
-
(S♂×R♀) .
Effect of insecticides treatments on certain biochemical
aspects of house fly Musca domestica
Effect on total protein content:
1- The highest level of protein concentration was detected in
Ismailia population (106±2.5 mg /gm tissue ) and F1
(104±2.5 mg /gm tissue), while the lowest level of protein
concentration was detected in Cairo population. Also the
total protein content of Fayoum was more than Cairo.
2- Polyacrylamide gel electrophoresis revealed that the total
protein of adult house fly M. domestica of Ismailia
population were separated into 19 bands ,Fayoum
population 17 bands and Cairo population 14 bands.
3- The highest number of protein bands occurred in F1
progeny and Ismailia population.
4- Two common bands (no. 46 and 52) were found in all
tested strains.
133
􀀉 Summary
Acetyl cholinesterase activity:
The results showed significantly higher AChE activity for
the field populations than laboratory strain. The highest AchE
activity was found in F1 and Ismailia population with 1.5 and 1.7
times respectively different from laboratory strain, the lowest
AChE activity was for Cairo population.
Effect on esterase patterns:
Effect on field and F progenies esterase patterns
The results showed significantly higher esterase activity
for the field populations than laboratory strain. The highest one
activity was found in Ismailia population and F1 with 3.20 and
2.5 times respectively differences from laboratory strain .
The native polyacrylamide gel electrophoresis (PAGE) of
esterase showed differences in esterase banding patterns between
laboratory and all field populations.
The highest number of esterase bands occurred in Cairo
population and laboratory strain (1-2-3-4-5).
Effectof Alkaline and acid phosphatase activity;
The alkaline phosphatase activity was expressed as ml/gm
134
􀀉 Summary
phenol released /individual /min. The result indicated that F1
-
progeny strain showed much higher levels of alkaline
phosphatase activity than all other population with 28.64
followed by F1progeny strain with 25, then Ismailia population
with 23.78 while laboratory strain had 4.16 U/L only.
Also, acid phosphatase revealed the same activity with
different population; Ismailia field population showed the highest
one with 61.2 U/L followed by F1, F1
-, Cairo, Fayoum and
laboratory strain respectively, with the values 56.8, 48.6, 42.1,
38.8 and 32.6 respectively